2018-Spring+PCB3063C-Prelab+quiz+2 (Co Home insert Design Layout References Mail

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2018-Spring+PCB3063C-Prelab+quiz+2 (Co Home insert Design Layout References Mailings Review View u .she X2 X2 A-L.. Paste i, 'd'-' coding reglon and coding r 2. In the construct DNA plasmid, what was not included? A. Replication of origin in bacteria B. Antibiotics resistant gene (such as Amp C Transposase 13. In this experiment, the reporter gene tells the experimenter exactly how much of the native protein was produced changes the color of the flies' eyes must be from the same type of organism as the organism into which it is being transferred is designed to be easily detected and reflect the activity of the native gene A. B. C. D. 14.After successfully incorporating into the genome of a fly, the construct DNA will A. change the functions of the native per gene B. cause the eyes to be white C. reflect transcriptional activity without interfering with functions D. alter the biological clock 15. How is light produced by the transgenic flies? A. The same way that light is produced in fireflies B. The transgenic flies have eaten a substance that produces light. C. Transcription of the transgene results in production of luciferase, which acts on luciferin. D. Turning on the room lights causes light to be produced by the flies. Page 1 of 1

Explanation / Answer

12.
Option C is correct.
A plasmid vector contains Ori, rop, selection marker and MCS.

13.
Option A is correct.
A reporter gene is used to identify the localization and to quantify how much product is synthesized.

14.
Option C is correct.
A reporter gene reflects the trascriptional function/regulation of a promoter.

15.
Option C is correct.
The reproter gene produces Luciperase product which is an enzyme that converts luciferin to a colourful product.

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