9. The following are described by what level of protein structure? Brief explain
ID: 188048 • Letter: 9
Question
9. The following are described by what level of protein structure? Brief explain your answer. a. Aspartate and lysine, located four residues apart from one another, b. A disulfide bond between two cystine residues, located at positions c. The covalent bonding between neighboríng glycine and alanine d. The hydrogen bonding between the side chains of a tyrosine hydrogen bonding within the polypeptide backbone. 34 and 276 along the polypeptide chain. residues residue located at position 15 in polypeptide chain A and a serine residue located at position 89 in polypeptide chain D e. The electrostatic attraction between a histidine residue and a glutamate residue at positions 17 and 34 along the polypeptide chain. 10.Below is a schematic representation of the active site of an enzyme with bound substrate. What type(s) of enzymatic catalytic mechanism(s) are taking place? Explain your answer. HO-R OH 0 5.5 AExplanation / Answer
a) aspartate and lysine, located ofur residue apart from one another, hydrogen bonding within the polypeptide backbone : Typical example of Secondary structure Alpha helix with (N-H) group of (i + 4)th residue hydrgen bonded to (C=O) group of (i)th residue, thus H - bonded within the backbone.
b) A di-sulfide bond between two cystine rsidues, located at position 34 and 276 along the polypeptide chain : The cystine residues are fur apart than normal alpha helix and beta sheet repeat. They need to come closer to form the bond. Thus, a tertiary folding level structure can be assumed and the formed di-sulphide bond will help to maintain the tertiary structure.
c) The covalent bond, neighbouring glycine and alanine residues : This covalent bondcan be guessed as to be peptide bond as glycine does not have any side chain rather H atom and alanine's side chain (-CH3) cannot form any more covalent bond with Glycine. Thus this can be at primary strcutre level of protein.
d) The hydrogen bonding between side chain of a tyrosine resdiue, located at postion 15 at polypeptide chain A and a Serine resdiue, located at position 89 at polypeptide chain D : Two different polypeptide chains are interactng here. Thus, this sub unit interaction can be termed as Quaternary structure level of protein.
e) The electrostatic attraction between a histidine residue and a glutamate residue at position 17 and 34 along the polypeptide chain : The electrstatic attraction is mainly a characteristic of a proteins tertiary structure level and the distance between two residue is longer than average distnace repeat of alpha chain and beta sheet. Thus we can assume the structre is at tertiary structre level.
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