method to overcome codon usage problems is the teas CHAPTERSO ntensive? a. Suppl
ID: 187754 • Letter: M
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method to overcome codon usage problems is the teas CHAPTERSO ntensive? a. Supply the genes for the rare tRNAs on a separate plasmid e the gene of interest, usually in the third position of the codon . Express the protein in the organism from which the gene originated. host cells that are expressing the protein longer so that they have more time to make the protein all of the above containing rare codons. e. in expression systems, s manufactured too quickly? a. activated AraC b. inclusion bodies c. T7 RNA polymerase d. functional proteir e. inactivated Lac repressor s. which of the fallowing forms when too much protein recombinant protein production be enhanced? 6. How can a. alteration of the C-terminal b. inclusion of PEST sequences c. add d. co-expression of a molecular chaperone e. all of the above sequence dition of a detergent to help prevent inclusion body formation z. What can be done to ensure a recombinant protein is excreted outside the cell? a. addition of a signal sequence b. using specialized export systems c. fusion to a protein that is normally exported d. expressing the protein in an alternative system, such as Gram-positive bacteria e. all of the above a. What could be used to increase the stability and purification of eukaryotic proteins from bacterial cells? a. a peptide tag b. a protein fusion c. a protease site d. PEST sequence e. a signal sequence 9. Why would it be necessary to express eukaryotic proteins in eukaryotic celils rather than in bacterial cells? a. Many eukaryotic proteins are modified and bacteria usually do not have b. Codon usage between eukaryotes and prokaryotes is differe c. Molecular chaperones are more readily available in eukaryotes. nt the enzymes needed for these modifications Protein production in eukaryotic cells never produces inclusion bodies which sometimes form in bacterial cells due to overexpression. e. none of the above Continued)Explanation / Answer
4) The correct option is Supply the genes for the rare tRNAs on a separate plasmid.
Supply the genes for the rare tRNAs on a separate plasmid, this method to overcome codon usage problems is the least labor-intensive. Less labor-intensive approach to the codon problem is to supply rare tRNAs. The tRNAs correspond to rare codons are in short supply.
5) The correct option is T7 RNA polymerase.
The T7 polymerase needs a double stranded DNA template and Mg2+ ion as cofactor for the synthesis of RNA. It has a very low error rate.
6) The correct option is Co-expression of a molecular chaperone.
Co-expression of a molecular chaperone can recombinant protein production be enhanced. Molecular chaperones are a ubiquitous family of cellular proteins, which arbitrate the correct folding of other target polypeptides. Molecular chaperones were co-expressed with anti-BNP scFv with the improvement of the production of functional anti-BNP in the cytoplasm of E. coli.
7) The correct option is All of the above.
Addition of a signal sequences, using specialized export systems, fusion to a protein that is normally exported and expressing the protein in an alternative system, such as Gram-positive bacteria all can be done to ensure a recombinant protein is excreted outside the cell.
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