toin sequence and other proteases ve within a pro either end o serine, cysteine,
ID: 187748 • Letter: T
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toin sequence and other proteases ve within a pro either end o serine, cysteine, threonine, or aspartic acid the" ctive sitos. of the following statement about mass spectroscopy is i onizes the sample and then meas d Proteases hydrolyze the peptide bond between amino acid contain metal ion cofactors within their acti ncorrect? the time it takes for the ions to ures 6. Which a. MS i and coential for aing protein profles of body fluids arg reach the detector h. SELDI-MS has great e. Glycosylation and phosp for horylation of proteins can be identified using Es in the future, be used to identify diseases before symptoms appear or MALDI. d. ESI is able to handle much larger ions than MALDI e. The time-of-flight for ions is directly correlated with the mass of the ion in Which of the following statements is not true about sequencing peptides with mass spectroscopy? a. The entire protein can be sequenced all at once using mass mass spectroscopy. spectroscopy b. Some purified proteins must be digested with proteases to eliminate undesirable characteristics such as hydrophobicity and solubility c. Two rounds of mass spectroscopy are used to determine the sequence. d. In order to determine the sequence, a pure sample of protein is obtained through 2D-PAGE or HPLC e. A database of protein ion spectra is used to compare the peaks of the unknown peptide to determine the sequence. 8. Which of the following is used to quantify proteins with mass spectroscopy? 2H b. 35S d. 32p 125 e. 9. Why are protein tags useful? a. Protein tags are exactly the same thing as reporter fusions and perform similar functions b. Tags allow the protein to be isolated and purified from other cellular proteins. c. Tags allow the protein to be quantitated d. Protein tags enable the protein to which they are fused to perform their function more readily. e. None of the above. o. How is biopanning useful to proteomics research? a. To express large amounts of protein on the cell surface of yeast b. To screen expression libraries in E. coli. c. To alter the cell membrane structures of cells by expressing foreign proteins on the cell surface.Explanation / Answer
1) The time-of-flight for ions is directly correlated with the mass of the ion in mass spectroscopy is the incorrect statement - Mass spectroscopy sorts the ions based on their mass-to-charge ratio. Mass to charge ratio is that when two particles with the same mass-to-charge ratio move in the same path in a vacuum when subjected to the same electric and magnetic fields.
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