r One type of protein array contains antibodies for se ts the proteome and cor C
ID: 187747 • Letter: R
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r One type of protein array contains antibodies for se ts the proteome and cor CH reening The other proteins attached by asrays may be screenesd for binding ilude enzye sulnstrates other proteins, and RNA n of a variety of labeled molecules to the newer field of research that looks at ct unding how cells . haiigr biodemical pathways in response in ihe .rwin ovelopment tetalbolites are analyzed using mass spectroscopy technisgues angrs in metabolites This method is during End-of-Chapter Ouestions s SDS used in the electrophoresis of proteins? ts the protein with a negative charge so that the sample can run a. SDS coats through the gel. s a specific protease that digests large proteins in the sample lows the coomassie blue stain to bind to the proteins in the gel so that they may be visualized. SDS adds more molecular weight to each sample so that the proteins do d. not run off the end of the gel. e. none of the above What is an issue with using 2D-PAGE? a. Hydrophobic proteins may not run as expected due to the hydrophobic surfaces. b. Highly expressed proteins may cover up proteins that are not as abundant but running in the gel nearby. Some proteins may not migrate through polyacrylamide and therefore not be represented on the gel d. Rare cellular proteins are hard to visualize with Coomassie blue protein stain. e. All of the above are issues with 2D-PAGE. a. Which one of the following is not used during Western blotting? a. secondary antibody with a conjugated detection system b. agarose gel electrophoresis c. non-fat dry milk d. primary antibody that recognizes the protein e. nitrocellulose membrane 4. Which of the following statements about HPLC is not correct? a. There are two phases to HPLC: mobile and stationary b. Separation, identification, and purification of proteins are just a few of the applications for HPLC. c. The downside to HPLC is that it is not very adaptable due to the justing the experimental conditions, changing the particle size of the sta- onary phase, and controlling temperature are factors that affect resolution. availability of stationary phase material e. All of the above statements are true. of the following is not an example of protease activity? me proteases cleave the phosphodiester bond between nucleic acid a. residues Continued)Explanation / Answer
1)NONE OF THE ABOVE
SDS IS THE DETERGENT AND Sodium dodecyl sulfate (SDS) to denature the proteins.
2) ALL OF THE ABOVE.
The disadvantages may include large amount of sample handling, less reproducibility, difficulty to separate low abundance proteins, acidic and basic proteins, very large and very small proteins and hydrophobic proteins.
3) AGAROSE GEL ELECTROPHORESIS.
Western blotting is the transfer of proteins from the SDS- PAGE gel to a solid supporting membrane.
4) all are true statement.
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