(1pt) CAP binds as a homodimer to specific DNA sequences upstream of genes invol
ID: 187545 • Letter: #
Question
(1pt) CAP binds as a homodimer to specific DNA sequences upstream of genes involved in the metabolism of sugar, but only when the protein is complexed with CAMP. CAP activates transcription by contacting RNA polymerase. 7. 1. 2. 3. Identify the regions of the protein that are responsible for dimerization, DNA binding, and cAMP binding. 5. 6. hd Dimerization (1), DNA binding (4), cAMP binding (3) c b. Dimerization (4), DNA binding (1), cAMP binding (3) t c. Dimerization (4), DNA binding (6), CAMP binding (5) bunits d. Dimerization (6), DNA binding (1), CAMP binding (2) 8. (1pts) The binding of cAMP to the CAP protein involves numerous electrostatic interactions. Most of the interactions are hydrogen bonds between cAMP atoms and the side chains of various amino acids in the CAP protein. Which of the following is a salt bridge'father than a hydrogen bond? a. between er1 of the opposite CAP monomer and a nitrogen of cAMP b. between Thr of CAP and a nitrogen of CAMP c. between Serof CAP and a phosphate oxygen of cAMP between GlubfCAP and a ribose oxygen of cAMPS$-+- e. between Arg of CAP and a phosphate oxygen of CAMPExplanation / Answer
7. Option b is the correct answer. Here region 4 is responsible for dimerization as it can be observed from figure only. This region clearly separate the two monomer of protein.
8. Option e is the correct answer. Salt bridge occurs between the side chain of arginine82 and a phosphate oxygen of cAMP.
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