Short answer. For each of the questions give a brief answer. If you feel that th

Question

Short answer. For each of the questions give a brief answer. If you feel that the answer requires a mathematical expression it is only necessary to put numbers is the proper places, you do not have to calculate the answer. 17. You make a cross using an Hfr strain of E. coli that is azi lys pen and an F strain that is az lys pen. Interrupted mating studies show that pen* enters the recipient last. In an interrupted mating between the same two strains, you select pen* recombinants and test b replica plating for the presence of the lys' and azi alleles. You score 300 individuals, and find the following genotype distribution: 259 azi lys pen1 az lyspen; 11 azik lys pen;29 azi lys pen*What is the gene order (give as first to enter... second...ast)

Explanation / Answer

Parental cross: aziR lys+ penR X azis lys- pens

It is given that penR enters the recipient first.

So, the genes that are closed to penR can enter the recipient more often than the genes that are farther away.

Progeny frequencies:

259 = aziR lys+ penR = The entire segment is transferred

1 = azis lys+ pens = Inturrupeted mating

11 = aziR lys- penR = Inturrupeted mating

29 = azis lys+ penR = Inturrupeted mating

Here, we need to look into the genotype frequencies to find out the gene order.

Greater the frequency, the closer the gene to penR.

Order of genes:

aziR- lys+ - penR

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