BIO 425 Team Based Learning Exercise 1 January 25, 2018 Participants: Priet Name
ID: 186560 • Letter: B
Question
BIO 425 Team Based Learning Exercise 1 January 25, 2018 Participants: Priet Name Prim Name Signature Signature Prim Name Name Signature Each group MUST consist of S members, and ALL participants must prim and sign above in order to receive crodit Each group will turn in ONE answer sheet before leaving class Part E: Multiple choice (2 points each). I. Which of the following units of measurement would he most appropriate for describing the size of an E cab colony? A) Centimeter Millimeter Micromeser D) Nanometer 2 If listing the following largest to smallest, which comoa? __ Flavobacterium johnsoniae, Epulopsclum ishelson, Paramecium, Influcnza virus D) Epulopincium fishelson; Flvebacterium johsoniae, Paramccium; Infloenza virus A) Polysaccharides found on the outside of eells 3 Which of the following structures might a basic dye bind so B) The DNA backbone The hydrophobic ragion of the ex toplasmic membrane D) A and B A, B and C Which ofthe following 15 TRUEoffluorescent molecules? 4 A) They absorb light at a specific wavelength B) They emit light a a specific wavelength C) They can be used to visualize either live or dead cells A and B A, B and C 5. Which of the following is TRUE of ABC transporters? A) They are only composed of two parts, a membrane-spaneing protein and an ATP-tydrolyzing protein B) They span both the cytoplasmic and outer membranes in Gram negative bacteria They can transport various organic and inorganic compounds, including trace metals A and C E) A, B and CExplanation / Answer
1. B ) Millimeter - A regular E.Coli colony is 3-6 mm in diameter.
2. B) Flavobacterium jhonsoniae- 2-5 um long, 0.3-0.5 um wide, Epulopiscium fishelsoni 700 um, Paramecium 250um, Influenza virus - 80–120 nanometers.
3. E) A, B and C. Basic dyes are positively charged ( Eg. Methylene Blue, Crystal Violet, etc.) as a result they will attract negatively charged molecules. Lipopolysaccharides which forms the outer membrane of gram-negative bacterial cell wall contributes negative charge to the cell wall hence the positively charged basic dyes will bind to the same.The DNA backbone is made up of phosphate ions which are negatively charged therefore the basic dye can efficiently bind. The hydrophobic region of the cytoplasmic membrane is made up of hydrophobic phospholipids, having phosphate head which is hydrophilic which can readily bind to the basic dye.
4. A, B, and C. Fluorescent molecules typically contain aromatic groups having several pi bonds, which helps in absorbance and emission of light. These fluorescent dyes are often used to determine the viability of cells (live or dead).
5. C. ABC transporters use ATP as an energy source and transport various substrates (Amino acids, peptides, glucose, etc.) and trace metals across the cell membrane.
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