n a PCR reaction, how does the concentration of the two primers compare to the c
ID: 186288 • Letter: N
Question
n a PCR reaction, how does the concentration of the two primers compare to the concentration of the target DNA?
The concentration of the primers is less than the concentration of the target DNA.
None of these is correct.
The concentration of the primers is greater than the concentration of the target DNA.
The concentrations are approximately equal.
A.The concentration of the primers is less than the concentration of the target DNA.
B.None of these is correct.
C.The concentration of the primers is greater than the concentration of the target DNA.
D.The concentrations are approximately equal.
Explanation / Answer
Ans. each primer should be between 0.1 and 0.5 µM and the required template for a standard reaction is 10 - 50 nanogram. So the concentration of primer is higher than the template as we require many copies of the target DNA which are formed by the annealing of new primer and then its extension.
However excess concentration also lead to the non specific binding of primers to the template DNA. So, an optimum concentartion need to be maintained.
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