Your Name: Week 3 Worksheet Instructions Write a short response (100-150 words)

Question

Your Name: Week 3 Worksheet Instructions Write a short response (100-150 words) to each of the following questions. Use your own words, and cite references where applicable i. You have isolated a strain of Salmonella that is resistant to Ampicillin, Chloramphenicol and Tetracycline. You observe that when you mix these cells with another strain of E. coli sensitive to the antibiotics, the sensitive strain becomes resistant. Provide an explanation for why this has happened. What are the implications of this observation? 2. (a) What does the following diagram represent? (b) What is the significance or meaning of the arrows between DNA and RNA, and the RNA and protein? (c) Are there exceptions to this rule? RNA DNA Protein s. Replicate the following double-stranded fragment of DNA. In your answer, indicate which strands are the new strands (mark with N) and which strands are the already existing strands (mark with O as shown below. HINT You should have 2 double-stranded fragments after replication. O: AGTACATTACGAC O: TCATGTAATGCTG

Explanation / Answer

Bacterial cross:This is the process of exchanging genetic material between two bacterial species.It is a kind of sexual reproduction.Then According to the question when salmonella species which is resistant to chloramphenicol,Ampicillin and tetracycline mixed with the sensitive strain of E-coli then it will also resistant to these antibiotics and the Ecoli is called as recombinant.

Recombinant: When One Organism contain other organisms genetic materials.

Let see what happen

ArArCrCrTrTr X A-A-C-C-T-T-

ArArCrCrTrTr

When selected on desired media all Ecoli were resistant to the antibiotic.

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