Question 2) You are simultaneously expressing three different proteins, A, B, an

Question

Question 2) You are simultaneously expressing three different proteins, A, B, and C, in the same cells – that of the bacterium E. coli. All three proteins contain a His-His-His-His-His-His peptide sequence covalently linked via a peptide bond to the native protein sequence. The polypeptides of all three proteins are ~630 amino acids long and have a pI of ~6.5. Protein A has a sequence Glu-Asp-Asp-Tyr –Asp-Gln-Gly sequence between the His-His-His-His-His-His and the native protein sequence. Protein B is known to dimerize (i.e. has a quaternary structure composed of 2 subunits), while proteins A and C are known to lack any quaternary structure. To start purifying these proteins away from all other proteins normally expressed in E. coli, you first lyse the cells and remove the insoluble cell debris. Based on what we learnt in class: A. What specific kind of chromatography (make sure to also mention the name of the material that makes up the chromatographic column) will you use to purify the mixture of A, B and C, away from all other E. coli proteins in the soluble lysate? (1 point)

Explanation / Answer

It is not mentioned that what you studied in your class. So i dont have all parameters.

In this experiement we will use high performance liquid chromatography.

In this we can use MCM14 molecule for stationary phase, sephadax beads and sometimes charged compounds.

In this chromatography we can seperate proteins with more accuracy.

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