108 Exarcise 12 DNA Extraction (avoid a. Pour the pea soup misture theough a cof
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108 Exarcise 12 DNA Extraction (avoid a. Pour the pea soup misture theough a coffe Sier or cheese closth into a small glas 4 Ad 2 tablepoons of liqguid dish detergent to the pea-soup misture and genty swin S. Let the soapy peas sit for 5 minutes on the lab bench to mix creating bubbles) in the glass beaker What does the soup do to the what pca-soup mixture? (Hint Think about the structure of the cell and components we will need to rupture to free the DNA.) 6. Pour the misnure into several tcs tubes, making sure to oaly fill cach one up 1/3 of the way 7. Add 1/8 teaspoon of meat tenderiaer into cach test tube and mix very gently (avoid creating babbles) 8. What purpose does the meat tenderizer serve in the DNA extraction? (Hint: Think about the con- densed structure of DNA in the nucleus that includes histone proteins 9. Tik each test tube and very slowly pour cold rubing alcohol down the side of the tube until you have a layer on top of the soapy peas. You'll need to add an equal amount of rubbing alcohol to the soapy peas What does the rubbing alcohol do to the soapy peas? (Hint: Think about water and its ability to act as a solvent on DNA.) 10. Let the test tube sit on the lab bench for five minutes. I1. Use a glass rod to pull out the long, stringy, white molecules you have created, being sure to stay away from the protein mass located at the bottom of the test tubes. If you don't see anything in the tubes, let it sit for up to an additional hour if time permits. You can also use the toothpick to search for any strands near the interface of the alcohol and the soapy-peas. What is the long, white, stringy molecule? Why can't you see a double-helix? Would thie DNA isolated from an animal cell look any different?Explanation / Answer
5.The soap (which contains enzymes)when added to the pea soap mixture helps to pull apart the lipids and proteins that are the main constituents of the membranes surrounding the cell and nucleus.When lipids and proteins are pulled apart,the membrane will be broken and dissolve so that DNA will be released from the cell.
8.DNA in the nucleus is associated with histone proteins to form complex condensed structure ,chromatin.In DNA extraction ,when meat tendizer is added,it functions as an enzyme that cuts away /helps in dissociation of histones f proteins from DNA so that DNA becomes free and unfolded.
9.Rubbing alcohol will help in the precipitation of DNA from the pea solution.
11.The long white thread stringy molecule is DNA.
We cant see the double helix because DNA extrcated is in mass and a single strain of DNA is very very small for us to see it with our naked eye.
No, the DNA isolated from animal cell would be no different.
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