(Please complete this by eight today! Thank you so so so much!!! These questions
ID: 183829 • Letter: #
Question
(Please complete this by eight today! Thank you so so so much!!!
These questions are based off the Peroxidase Enzyme Lab.
Data:
Questions:
All of Opportunities of Inquiry #1:
Pictures of Lab for Reference:
Once again, Thank you so much for your time!
Time Trial 1(nm) Trial 2(nm) Trial 3(nm) 20 0.05 -0.03 0.07 40 0.06 -0.02 0.09 60 0.06 0.00 0.09 80 0.07 0.02 0.10 100 0.09 0.03 0.10 120 0.10 0.05 0.10 140 0.11 0.07 0.11 160 0.12 0.09 0.11 180 0.13 0.10 0.11 200 0.14 0.12 0.12 220 0.15 0.13 0.12 240 0.15 0.15 0.13 260 0.16 0.17 0.13 280 0.17 0.18 0.13 300 0.18 0.20 0.14 Opportunities for Inquiry 1. Consider the following questions while reflecting upon your knowledge of enzyme structure, reaction rates, and how inter- actions among proteins as well as biotic and abiotic factors may influence the rate of an enzyme-catalyzed reaction. a. What evidence from the effect of enzyme concentration on the reaction rate supports a dynamic theory for biological reactions? b. Does the substrate concentration influence the rate of the reaction? c. How might a model of enzyme-substrate binding be reflected in the shape of the curve for substrate concentration ver- sus reaction rate? d. Does enzyme activity depend on pH? Is there an optimum pH for the reaction? e. How does the pH profile for an enzymatic reaction demonstrate the acidic and basic properties of proteins and enzymes? f Do metal ions activate or inhibit the rate of enzyme-catalyzed decomposition of hydrogen peroxide? &What; factors might predict the effect of temperature on the rate of an cnxyme-catalyzed reaction? h. Do peroxidase enzymes from different vegetables have similar activity?Explanation / Answer
ANSWER: a) Enzymes are biological catalysts which affects the rate of reactions, when enzyme concentration increases,the substrate concentration also increases by which the rate of reaction also increases.
b) yes, as the substrate concentration increases, the rate of reaction also increases.
c) A hyperbola curve graph is obtained between when enzyme substrate binding occurs in substrate concentration vs reaction rate plotted in a graph.
d) yes, enzyme activity is pH dependent, where change in pH of an enzyme can alter the shape, conformation as well as active binding sites of the enzymes which may affect functionality of the enzyme. Every enzyme has its own specific optimum pH for each specific reaction types.
e) pH of an enzymatic reaction gives the information about the ionic status of the amino acids involved depending on this protonation ability, the amino acids forming the proteins and enzymes can be either acidic or basic in nature.
f) As Hydrogen peroxide is stable mostly when it is in its undissociated form, so it may get decomposed when presence of metal ions is there, thus these ions activate its decomposition reaction.
g) the velocity and kinetic energy predict the rate of reaction when temperature is involved.
h) yes, peroxidase activity in different vegetables is different as it depends on the biochemical structure, composition, kinetic energy, specific heat etc which are not same in all vegetables.
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