23. Transcribe the following sequence of DNA located on the coding strand: 5’-AC

Question

23. Transcribe the following sequence of DNA located on the coding strand: 5’-ACGGTACATT-3’

a. 5’-ACGGUACAUU-3’

b. 3’-ACGGUACAUU-5’

c. 5’-UGCCAUGUAA-3’

d. 3’-UGCCAUGUAA-5’

I thought the answer was A (even though the answer wasn't marked correct), and someone here told me the correct answer is B. However, I researched further into the matter and found these two sources, which are saying it should be A (the mRNA is the same exact code in the 5' 3' direction, only with U replaced for T)

So, if these are the right processes, why isn't the answer A then? I don't know what to believe anymore.

DNA coding strand 5' A T C C T G G T A G A T C C T T A A G DNA transcribed d 3' T A G G A C C A T C T A G G A A T T m-RNA 5' A U C C U G G U A G A UC C U U A A G transcription Fig. 4. 1. Comparison of the DNA coding strand, the DNA transcribed strand and m-RNA. m-RNA is similar to the coding strand except that T is replaced by U. Transcription occurs from the transcribed strand in the 51 to 3' direction

Explanation / Answer

All of the RNA in cell made by DNA transcription. RNA molecule is complementry to the one strand of DNA, this strand is called template strand. Sequence of mRNA is similiar to the other strand that is coding strand of DNA. RNA strand is extended by one nucleotide in 5' to 3' direction.

Here the DNA sequence located on the the coding strand

5' ACGGTACATT 3' coding strand

3' TGCCATGTAA 5' template strand

5' ACGGUACAUU 3' mRNA strand

Your answer is right ( A) according to this explanation because mRNA always transcribed 5' to 3' direction. mRNA consist of the codon corresponding to those present in the coding strand of DNA except instead of T, U base present in mRNA. RNA polymerase catalyze the transcription process always in 5' to 3' direction.

However there is a one possibility, that the position of this sequence of coding strand that change the polarity of mRNA strand synthesize from template strand because the difference in the option A and B , is the difference of polarity.

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