D) 36% E) 56% 60. A recently isolated bacterial strain has been given to you for
ID: 180998 • Letter: D
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D) 36% E) 56% 60. A recently isolated bacterial strain has been given to you for general characterization of the genome. You begin by isolating the DNA and subjecting it to thermal denaturation. You use Escherichia coli (E col) DNA as a reference. 1.2 GN a 1.0 70 80 90 100 Temperature C Ecoli unknown organism Based on the information in the graph, you can determine that the DNA of the new strain has a high A) G-C content with respect to E coli. B) pyrimidine content with respect to E coli. C) A-T content with respect to E. coli. A) 61. Alteration of histones by addition of methyl and acetyl groups to particular amino acids changes B-DNA to Z-DNA. B) results in the remodeling of that can activate or inhibit gene expression. creates G bands chromatin of chromosomes. seen after Giemsa staining D) helps preserve telomeres during DNA replication. E) alters the denaturation and renaturation of DNA. 62. Fluorescent in situ hybridization (FISH) would be helpful in determi A) the location of B-DNA Z-DNA. iningExplanation / Answer
60. G-C content is high with respect to E.coli
Since energy required to break bonds between GC is more, as it has three bonds when compared to AT has two. If GC is more it implies bonds between them is more, so the more energy in form of more temperature.
61. Results in remodeling of chromatin that can activate or inhibit gene expression
Basing on which amino acid is methylated, it leads to activation or inhibition.
73. DNA replication is semi conservative
74. D eamination of 5-methylcytosine forms thymine.
Leading to conversion of cytosine to thymine causing mutation also known as “Transition mutation”.
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