Recent advances in genome engineering technologies based on the CRISPR-associate
ID: 180488 • Letter: R
Question
Recent advances in genome engineering technologies based on the CRISPR-associated RNA-guided endonuclease Cas9 are enabling ... DNA sequences within the endogenous genome and their functional outputs are now easily edited or modulated in virtually any organism of choice. Describe the mechanisms of how CRISPR/CAS9 allows for the editing of a host genome. Describe an application of CRISPR/CAS9 that is not disease related. Describe the ethical and technical concerns of the system. Chapter 7 Essay Questions You have a patient that exhibits the beginning symptoms of neurological loss of function and decide to sequence the patient's genome to characterize any genetic markers for disease. You notice a mutation in prion (PrP) protein's GPI anchor domain. Describe what mutations would likely cause loss of function (in relation to the GPI anchor and the PTMs of PrP). Describe how you would characterize any problems in the patient's carbohydrate using biochemistry-based assays. You decidExplanation / Answer
CRISPR-Cas9 is an advanced molecular technology that is used to edit parts of genome. It is used to remove, add, or alter the portions of DNA sequence. It is simplest, precise, and versatile method of genetic manipulation. The system consists of two molecules that can mutate or alter the DNA. The two components are
CRISPER stands for clustered regularly interspaced short palindromic repeats. CRISPERs are segments of bacterial DNA and CRISPER/Cas system provides immunity to the prokaryotic cells.
The following is the mechanisms of CRISPER-Cas9 system:
Mature crRNAs assoacited wit Cas proteins to form intermediate complexes
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