To follow up on a microarray data you generated on non-myeloma skin cancer tumor

Question

To follow up on a microarray data you generated on non-myeloma skin cancer tumors, you want to determine whether the Myc transcription factor binds to the promotes of the genes you identified in your microarray. You decide to perform a ChIP assay.

1. What antibodies will you need to perform this experiment? Make sure to include antibodes for a positive control.

2. To which portion(s) of the genome would you design PCR primers in order to perform this experiment? Make sure to include primers for both a positive and negative control.

Explanation / Answer

Exmaples of antibodies :

Human SOX17 Antiboby :

Clonality: Polyclonal

Host: Goat IgG

Applications: ChIP, ICC/IF, Simple Western, WB

Human / mouse/rat SOX2 Antibody :

Clonality: Polyclonal

Host: Goat IgG

Applications: ChIP, ICC/IF, Simple Western, WB

Human / mouse/rat SOX2 Antibody :

Clonality: Polyclonal

Host: Goat IgG

Applications: ChIP, ICC/IF, Simple Western, WB

Human/mouse Brachyury Antibody

Clonality: Polyclonal

Host: Goat IgG

Applications: ChIP, ICC/IF, IHC, WB

Human Olig2 Antibody

Clonality: Polyclonal

Host: Goat IgG

Applications: ChIP, ICC/IF, IHC, WB

Portion(s) of the genome would you design PCR primers in order to perform this experiment

Here are some general rules for designing ChIP primers:

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