24. Shown is a representation of the results of an electromobility shift assay (

Question

24. Shown is a representation of the results of an electromobility shift assay (EMSA). The plus and minus symbols indicate which of the listed components were included in each shift assay. You know from other experiments that both ZF and SWI/SNF are required for expression of your favorite gene (YFG). Based on the data shown, propose a model as to how YFG is activated in vivo. In your answer, indicate a general role of a transcription activator and a chromatin remodeler. (6 pt) Naked DNA-labeled DNA fragment Naked DNA t t t t containing regulatory element Mono nucleosome Mononucleosome same DNA fragment assembled around ZF histone octamer AZF ZF zinc finger protein SWI/SNF AZF ZF with zinc finger deleted Sample wells SWI/SNF chromatin remodeling complex End of Gel

Explanation / Answer

Expression profile of VCAM-1 ( Vascular cell adhesion molecule 1) and E-selectin in mouse can be studied after administering them with lipopolysaccharide. Complementary DNA probes for VCAM-1 and E-selectin are prepared and after conducting Northern blot analysis, one could see increased level of transcription in in lung, heart, and kidney for endothelial cell adhesion molecules.

Transcription activator promotes the functioning of RNA polymerase, which performs the function of transcription.

Through chromatin remodelling, chromatin is dynamically modified which makes it possible for the condensed genomic DNA to gain access to the regulatory transcription factors.

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