10) in SDS-PAGE, how would removal of the SDS from the gel affect the protein\'s
ID: 179521 • Letter: 1
Question
10) in SDS-PAGE, how would removal of the SDS from the gel affect the protein's mobility? (3pts) not all proteins would enter the gel because some proteins will have a positive charge not all proteins would enter the gel because some of the proteins will have a negative charge the proteins would not move because the SDS is necessary to conduct current through the gel the proteins would still move in the gel as before, just slower oligonucleotides? Western blot Eastern blot Southern blot Northern blot In the Rao paper, why did the authors use HeLa cells to isolate (pts) IKK? the cells express lots of IKK. the activity of IKK in HeLa cells is easily inducible the cells are easy to grow and maintain in a lab All of the aboveExplanation / Answer
Answer:
10). not all proteins would eneter the gel because some proteins will have a positive charge
For proteins, sodium dodecyl sulfate (SDS) is an anionic detergent applied to protein samples to linearize proteins and to impart a negative charge to linearized proteins as they are positive. This procedure is called SDS-PAGE.
11). Southern blot
A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization
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