I was trying to hit blast on ncbi site.. but I cannot.. so Primer mix A contains

Question

I was trying to hit blast on ncbi site.. but I cannot.. so

Primer mix A contains primer 1 and 2 and will copy the first half of the gene.

Primer mix B contains primers 3 and 4 and will copy the second half of the gene.

so what's the product length for both primer mix A and mix B ??

Primer 1 6 TCAAC GTG CTG CCC ACC AAG Primer 2 (Blue reverse) 5 GGC TGTGCCGTGAATGAGGGG. Primer 3 (blue): 5 CCCCTCAT TCACGGCACACGG Primer 4 (green, reverse): 5 AGGCGTCTcCTGATAGACCGCA CA ACGTGCT GCC (CACCAAGATAT CCGTCCTT TGAAGA TTT TAATTTTAAATCT CATGC CACAGAAAATGGTCACAGAG ACCCAGITTG TTGCGCCACTT(GGCTAATACACCCCTACAA CTGGAT ATT GATT TTCTCTA TATGGA (GAGCCACC GTTCTAAAACAA CTCGT TCAGAGCA. CATGGAGAC CTT CTATAAAACTT TTC (CTGAAGTCAAGGATGAGTATTT TGATGGGATGA TCATCACGG GTGCTCCAGTTGAGCATTTACCATTTGAGGAAGTGGACTATTGGGAGGAA TTTAGACAGATG(CTTGAGTGGTC TTAAGACTCATG TCTATT(CGACCCTTCATAT CTGTTG GGGGGC TCAGGCT GGGCTTT ATCTGC (CCTT ATGGTGTAG TAC CAG ATGGACAGTA A(GCTATCAGGTATTT ATCCTCAGGACACC CTAAAAGAGGGT CACCTTCTATTTAGAG GC TTTGAT GATA GCTATGT ATT CACGGCACACGGAG ATTTCTLAAGGAAGAGGTCTTAAA CA AGACCAATC TCGAGATTTTATCAGAAGGA CCTCAGGTTGGGGT TTCTATTTT GGCCAGT CGTGATTTACGAGAAATTTA TAG TTTTTGGT CATT TGGAGTATGACCGTGATACC TTG GC AAAAGAGTA TTTTCGLAGATC GTGATGCAG GTTTCGACCCACAT ATTCCAGAAAAATTACT TTAAAGATGATGATGTTAATCAGGTA (CCTTGTCT TTGTTG(GTCTTCAT CTGCA GCCCTC TTTTTCAGTAATTGGGTAGACCATT GCGGTCTATC AGG AGACGCC

Explanation / Answer

The specificity of the ampilicon will depend on primer 1 and primer 4 as primer 2 & 3 are practicaaly same (complementary in sequence).

So if it is seen in that light, the first amplicon for primer 1 & 2 will be ~512 bp.

The second amplicon will be ~327 bp.

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