Primer mix A contains primer 1 and 2 and will copy the first half of the gene. P

Question

Primer mix A contains primer 1 and 2 and will copy the first half of the gene.

Primer mix B contains primers 3 and 4 and will copy the second half of the gene.

what size band (in base pairs) would you expect to see on a DNA gel after you conducted PCR with these two primer sets?

Primer 1 6 TCAAC GTG CTG CCC ACC AAG Primer 2 (Blue reverse) 5 GGC TGTGCCGTGAATGAGGGG. Primer 3 (blue): 5 CCCCTCAT TCACGGCACACGG Primer 4 (green, reverse): 5 AGGCGTCTcCTGATAGACCGCA CA ACGTGCT GCC (CACCAAGATAT CCGTCCTT TGAAGA TTT TAATTTTAAATCT CATGC CACAGAAAATGGTCACAGAG ACCCAGITTG TTGCGCCACTT(GGCTAATACACCCCTACAA CTGGAT ATT GATT TTCTCTA TATGGA (GAGCCACC GTTCTAAAACAA CTCGT TCAGAGCA. CATGGAGAC CTT CTATAAAACTT TTC (CTGAAGTCAAGGATGAGTATTT TGATGGGATGA TCATCACGG GTGCTCCAGTTGAGCATTTACCATTTGAGGAAGTGGACTATTGGGAGGAA TTTAGACAGATG(CTTGAGTGGTC TTAAGACTCATG TCTATT(CGACCCTTCATAT CTGTTG GGGGGC TCAGGCT GGGCTTT ATCTGC (CCTT ATGGTGTAG TAC CAG ATGGACAGTA A(GCTATCAGGTATTT ATCCTCAGGACACC CTAAAAGAGGGT CACCTTCTATTTAGAG GC TTTGAT GATA GCTATGT ATT CACGGCACACGGAG ATTTCTLAAGGAAGAGGTCTTAAA CA AGACCAATC TCGAGATTTTATCAGAAGGA CCTCAGGTTGGGGT TTCTATTTT GGCCAGT CGTGATTTACGAGAAATTTA TAG TTTTTGGT CATT TGGAGTATGACCGTGATACC TTG GC AAAAGAGTA TTTTCGLAGATC GTGATGCAG GTTTCGACCCACAT ATTCCAGAAAAATTACT TTAAAGATGATGATGTTAATCAGGTA (CCTTGTCT TTGTTG(GTCTTCAT CTGCA GCCCTC TTTTTCAGTAATTGGGTAGACCATT GCGGTCTATC AGG AGACGCC

Explanation / Answer

you must use the Blast Tool https://www.ncbi.nlm.nih.gov/tools/primer-blast/ to find the size of PCR product. Just paste the sequence in the correct order. You will get the following results https://www.ncbi.nlm.nih.gov/tools/primer-blast/primertool.cgi?ctg_time=1481507507&job_key=w8kc0wcUCrwthhyDEeM4sWv4KYNG6zKeRw

The Product length = 599 bp.

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