5. (8 points) Your laboratory is investigating the effects of a novel anti-cance

Question

5. (8 points) Your laboratory is investigating the effects of a novel anti-cancer drug on genome- wide mRNA levels in human prostate cancer cells. You decide to perform a preliminary experiment using a custom cDNA microarray with probes for 6 genes. The background- subtracted fluorescence intensity data obtained from the microarray experiment is shown in the table below. Assume the untreated sample is the control, and the treated sample is the experimental sample. Control Drug (Cy3). Gene Name Drug (Cy5) p53 258 Rb 570 145 28 ld1 230 183 Actin 435 419 807 Tubulin 751 a. (3 points) Use the Global Intensity Normalization method to calculate a normalization factor 3 (Experimental/Control for this experiment. 0,977 aaa5 b. (3 poin) Using the Global Intensity normalization factor, write the normalized intensities for the p53 gene in the table below: Gene Name Drug (Cy3) Drug (Cy5) p53 101 You repeat the microarray experiment three additional times, and obtain the following normalized intensity data for the Myc gene:

Explanation / Answer

In global intensity normalization factor method makes an assumption about data, and the assumption is that number of majority spots on your array are expected to see a ratio of main experiment and in control it is divided by 1.

Based upon this information for p53 (Cy5) showing 258 and the number of arrays are 6and combining this two values we will get 264 and for control it is divided by 1 we will get the same value.

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