LAB= Trypsinizeiton of RAW246.7 Macrophage cells and confluence Objective = The
ID: 178836 • Letter: L
Question
LAB= Trypsinizeiton of RAW246.7 Macrophage cells and confluence
Objective = The purpose of this excercise is to learn how to remove adherent cells from a growth plastic surface. the removal of adherent cells from growth surfaces is required in cell culture in order to count cells for reseeding to perform future experiment or for cell passaging.
QUESTION
1. What is the biosafety level required when using these cells and what does it mean? What is the morphology of RAW cells under the microscope prior and post trypsin treatment?
Explanation / Answer
RAW 246.7 is a cell line obtained from tumor induced by murine leukemia virus so that care has to be taken while performing experiment. Gloves should be used so that metabolite cannot be touched by hand directly.Sterile condition should be maintain in the lab , proper disinfectant should be used for glass wares used in experiment.
After trypsinization cell become separated from each other and can be seen more clearly
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