26. A recent study shows that the siRNA knockdown of BRAF expression slows the g

Question

26. A recent study shows that the siRNA knockdown of BRAF expression slows the growth of melanoma cells in culture. The BRAF protein was targeted because it is known to be abnormal in most melanomas. Which should have the greatest knockdown effect?

a. Introduction of antisense RNA, exactly complementary to BRAF mRNA

b. Introduction of antisense RNA, almost complementary to BRAF mRNA

c. Introduction of sense RNA, that matches BRAF mRNA

d. Introduction of dsRNA consisting of BRAF sense and antisense RNA

Explanation / Answer

a. Introduction of antisense RNA, exactly complementary to BRAF mRNA

RNA that is complementary to the mRNA interest will form dsRNA to which ribosomes cannot bind hence translation will be inhibited. In this type of  siRNA knockdown exactly complementary siRNA will show the greatest effect.

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