29. A cultured batch of human kidney cell has 5,000 copies of ALR mRNA/cell. You

Question

29. A cultured batch of human kidney cell has 5,000 copies of ALR mRNA/cell. You injected some synthetic dsRNA with sequence homology with the ALR mRNA (average 2 copies/cell). After an hour, there was no ALR mRNA in the cell. How the RISC managed to destroy all ALR mRNAs using only two copies of the siRNAs.
A. The cells generated additional dsRNA by using RdRP.
B. The siRNA activated nucleases that nonspecifically destroyed all RNAs.
C. The RISC complex might have used the guide RNA repeatedly and cut the target like an enzyme

30. Which of the following is roadblock to widespread use of RNAi for cancer therapy?
A. Synthetic dsRNAs are unstable.
B. Inserting dsRNAs inside the target cells.
C. Toxicity of the synthetic dsRNAs
D. All of these

Explanation / Answer

Answer:

29.

B. The siRNA activated nucleases that nonspecifically destroyed all RNAs.

30. All of the above

i.e A. Synthetic dsRNAs are unstable. B. Inserting dsRNAs inside the target cells. C. Toxicity of the synthetic dsRNAs

D. All of these is answer

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