(show all your works and measure that you answer all questions for part a to par
ID: 178110 • Letter: #
Question
(show all your works and measure that you answer all questions for part a to part d below. Sorry for long questions, but I give thanks to anyone who can help me)
Note: Please respond to all questions for part a to part d below. Thank you
The lac operon is found in the DNA of Escherichia cell (such as the E. coli found in our intestines Scenario: you drink milk containing lactose after not having eaten all day (i.e. your blood glucose levels are low) a. Is the repressor protein active or inactive? What caused the repressor to become active or inactive? b. Is the activator protein CAP active or inactive? What caused CAP to become active or inactive? c. Will transcription of the gene occur? d. A point mutation in the operator region of the lac operon prevents the binding of enzymes and proteins to the operator. How will this affect the activity of the operon?Explanation / Answer
a. lac repressor senses lactose. In absence of lactose, repressor blocks the transcription of the gene while in presence of lactose, lac repressor binds to lactose and allows transcription.
Once a person drink milk containing lactose, lac repressor will sense lactose and binds to lactose to become INACTIVE.
b. ACTIVE
In the given case (presence of lactose and absence of glucose), cyclic AMP (cAMP) joins with a catabolite activator protein (CAP) that binds to the lac promoter and facilitates the transcription of the lac operon.
c. Yes, the transcription of gene will takes place.
d. In the absence of binding of proteins and enzymes on the operator, the gene regulation will not take place. As a result, higher levels of the encoded proteins will be produced.
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