I am doing a presentation on the \"DNA Binding and Cleavage by Human Parvovirus
ID: 178045 • Letter: I
Question
I am doing a presentation on the "DNA Binding and Cleavage by Human Parvovirus B19 NS1 Nuclease Domain" biochem lab report. part of the report talks much about stoichiometry but i do not understand what it means. i remember stoichiometry being the conversion of moles with reactions. my question is what is the definition of the stoichiometry that is talked about in this lab report.
Stoichiometric Measurements of DNA Binding Using the Gel Shift Method. Measurement of the stoichiometry of binding of NS1-nuc to various DNAs was performed using the gel shift assay as described above but with 10 HM 32 DNA, and NS1-nuc concentrations of S240 uM. Gels were imaged as described above, and the stoichiometry was determined from the break or end point in NS1-nuc concentration after which no further binding occurred: the percent bound versus NS1-nuc concentration was fit to two straight lines, one before and one after saturation of binding of the DNA, and their intersection used to determine the concentration of NS1-nuc that fully saturated the 10 uM DNA in the assay. Examples of gel images and data fitting are given in Figures S5 and S6.Explanation / Answer
In this laboratory report they are talking about the stoichiometry of an agent binding with biological molecular structures. In biology it is very tough work to count each and every atoms related stoichiometry. So they focused upon the concentration of an agent binds or no bound. There is threshold level also where an agent cannot bind further to biological molecules. This is happened because the covalent structures of biological molecules.
So by using the values of binding, no binding they create plots and charts to make under stable the particular agent’s behavior, for example enzyme substrate catalysis reaction analysis.
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