used as a sis buffer 5. Enlist the significance of following reagents: (4) a) Ca
ID: 177907 • Letter: U
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used as a sis buffer 5. Enlist the significance of following reagents: (4) a) Calcium Chloride b) Antibiotics: It is used to event the gro bacteria c) Heat Shock: d) Ethanol Treatment: e) SDS 6. Compare between efficiency of two 1 20-100 and 2) you expected to draw so microliter (Hu) of any solution. For three times you drew fluid, you observed that 1) and c) 54.85 ul. Calculate the average difference of observed minus expected weighs between the two pipettes. Which one is more suitable for use? Show your work. (5) 20 200 20-woo 7. what will happen if you forgotto use chloroform during DNA extraction? (1) u forgot to use chloroform during the DNA atraction gou wont be able to Pick up DNA rescuts because hard to pick up with the out chlordommExplanation / Answer
Ques-5:
C. Heat shock is the procedure of subjecting cells to higher body temperature than the ideal body temperature as a result cells always trying to resist by transcriptional upregulation of heat shock proteins. When a cell is under heat stress or heat shock and it can exert severe denaturation action on plasma membrane. Therefore, a higher content of unsaturation has leading to lower resistance to heat.
d. Ethanol is the denaturing agent used to kill the live bacterial cells result in exposure of internal contents to the outside during ethanol treatment
e. SDS (Sodium dodecyl sulfate): it is an anionic detergent used to lyse the cells during DNA extraction and protein purification
Ques-9:
MW --> 92.211
For 1M solution ---> weigh 92.211 grams of MgCl2 using simble balace ---> pour into a long necked 1000ml flask & dilute with solvent (distilled water) till to 1000ml (1litre)
For 4M solution ---> weigh 4 x 92.211 grams of MgCl2 --> 368.84 grams of MgCl2 using simble balace ---> pour into a long necked 1000ml flask & dilute with solvent (distilled water) till to 1000ml (1litre)
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