Please answer the following genetics questions: 10. What is the general primer u

Question

Please answer the following genetics questions:

10. What is the general primer used in RT-PCR? Why?
12. EcoRI a 6-cutter. Theoretically, how often does it cut in a long stretch of DNA given that the GC content of the organism we study is 60%?
Please answer the following genetics questions:

10. What is the general primer used in RT-PCR? Why?
12. EcoRI a 6-cutter. Theoretically, how often does it cut in a long stretch of DNA given that the GC content of the organism we study is 60%?


10. What is the general primer used in RT-PCR? Why?
12. EcoRI a 6-cutter. Theoretically, how often does it cut in a long stretch of DNA given that the GC content of the organism we study is 60%?

10. What is the general primer used in RT-PCR? Why?
12. EcoRI a 6-cutter. Theoretically, how often does it cut in a long stretch of DNA given that the GC content of the organism we study is 60%?

Explanation / Answer

10. During RT-PCR mRNA is converted into cDNA. This involves the use of general primers oligo dT. mRNA contains poly A tail at the 3 prime end. Thus oligo dT primers form complimentary pairing with these residues and further elongation is carried out by reverse transcriptase enzyme.

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