Figure 10 shows immunostaining of a polytene chromosome spread with an antibody
ID: 177435 • Letter: F
Question
Figure 10 shows immunostaining of a polytene chromosome spread with an antibody against a chromosomal protein. A color version of this photomicrograph will be projected on the screen during class. How would you describe the immunolocalization pattern of this protein ? Which region of the chromosome is most highly stained with the Texas Red-labeled antibody ? Its staining the centromeric heterochromatin Based on this localization pattern and what you learned in Chapter 12 about constitutive heterochromatin surrounding centromeres, what protein do you think is recognized by the antibody? HP1 Are there any other proteins (or covalentty modified form of histone) you would expect to have a similar staining pattern? Lysine 9-methylated histone H3 Careful examination of the immunostaining pattern reveals a few minor sites of staining on the chromosome arms. Speculate what the constitutive heterochromatin protein is doing at these sites. Don't be afraid to speculate. Your hypothesis is as good as anyone else's.| Briefly describe one experiment you could use to test your hypothesis.Explanation / Answer
Ques-11:
Polytene chromosomes are giant over sized chromosomes found in the salivary glands of Drosophila. Polyteny defined as the alignment of duplicated chromosomes in parallel. Polytene chromosomes possess a joint centromere called as chromo center.
Careful examination of the immunostaining pattern reveals a few minor sites of staining on the chromosome arms. The constitutive heterochromatin protein is “transcriptionally active” therefore, RNA transcription is occurring at these sites, with chromosomal puffs. This is excellent visualization (with light bands) of immunolabeled banding patterns in polytene chromosomes mainly with transcriptionally active heterochromatin. Dark band s are transcriptionallly inactive heterochromatin
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