What is the common plasmid above known as? Shuttle vector Expression Vector Clon

Question

What is the common plasmid above known as? Shuttle vector Expression Vector Cloning vector Yeast artificial chromosome (YAC) In the plasmid above, what is the purpose of the Amp gene? To determine whether or not your gene of interest was properly inserted into the cloning site. This is the site where you would cut with restriction enzymes and insert your gene of interest. This is for the cell know where to begin replication of the plasmid This is a resistance gene so that only the cells that have the plasmid will live in the presence of ampicillin in the plasmid above, what is the purpose of the lacZ gene? To determine whether or not your gene of interest was properly inserted into the cloning site. This is the site where you would cut with restriction enzymes and insert your gene of interest. This is for the cell know where to begin replication of the plasmid This is a resistance gene so that only the cells that have the plasmid will live in the presence of ampicillin

Explanation / Answer

All the answers (including those for questions 19 and 20) have been correctly identified by you.

Question No. 19: The purpose of the Amp gene is indeed to allow selection of the successful transformants. The media in which the cells will be grown after transformation will contain Ampicillin and only those cells that will carry the plasmid (which in turn is carrying the Ampicillin resistance gene, Amp) would be able to grow in this selective medium.

Question No. 20: The purpose of the lacZ gene is indeed to determine whether or not the gene of interest has been properly inserted in the cloning site. The lacZ gene in the plasmid produces a part (-peptide) of the enzyme -galactosidase, which is required by the host to convert X-gal into a blue colored pigment; the host carries a defective -galactosidase and the -peptide is needed to restore its activity. Successful insertion of the gene of interest in the cloning site of the plasmid disrupts the lacZ gene and -peptide is not produced. Plasmids bearing such inserts, when transformed into the host, are not able to complement the host’s defective -galactosidase and hence, the host is not able to convert X-gal into the blue colored pigment.

Related Questions

Navigate

• Browse (All)
• Browse W
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.