FRESNG STATE BIOL 104 LAB EXAM (Fall 2015) This exam is worth so points (400 of
ID: 175963 • Letter: F
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FRESNG STATE BIOL 104 LAB EXAM (Fall 2015) This exam is worth so points (400 of your final grade) What is the typical ratio of males to hermaphrodics in wild type populations of C. Elegans 1000 2. Agarose gel a. Moves the DNA through the gel from a cathode (-)to an anode b. zes a molecular weight ladder to quantify the size of the DNA product Separates size d All of the above None of the above component of the PCR mastermix acts as the building blocks to make a new DNA 3. Which a. DNA template b, Primers Taq polymerase e. None of the above 4. Which of the following is a part found on a microscope? a a. Lens stabilizer lb. Condenser lens optical reverberator d. All of the above e. None of the above 5. Which of these conditions can lead to nematodes entering the Dauer stage? a. Crowding b, Starvation High temperature All of the above e. None of the above w har process leads creation er males in merialodes? microscopes because 7, Electron microsoopes have a advantage allow the to esamine living cells. b electron microscopes have a larger obiective aperture are easier to use. the wavelength of electrons is shorter than that of light e, electron microscopes allow whole cells to be viewed. s. Which ofae following microscopy techniques telies on he dyes or any with the wavelength of light to produce a high contrast image without the need for c) Transmission Electron microscopy ITEM) e) None of the above Phase-contrast microscopy is a fluorescence-based microscopy technique. b) False A good definition for magnificationis apparent sze of an image dividedby its real sze. Resolution is the minimum distance between two points that can still be sen as distinct b) False 12. Contrast is the difference between the brightness ofvarious parts ofthe specimen compared to that of the background. b False 13. Which of the following differentiates between independent assortment and segregation? A) The law of independent assortment requires describing two or more genes relati B) The law of segregation requires describing two or more genes relative to one ano The law of segregation requires having two or more generations to describe. D) The law of independent assortment is accounted for by observations of prophase The law of segregation is accounted for by anaphase of mitosis.Explanation / Answer
2. d) Agarose gel electrophoresis moves DNA through the gel from cathode to anode and separate DNA based on their sizes with the help of molecular weight ladder.
3. d) Taq polymerase, a heat stable enzyme adds deoxinucleotides to DNA template
4. b)
5.d) nematodes enter Dauer stage in harsh environmental condictions like crowding, starvation, and high temperature
6. c)
7. d)
8. a) fluorescence microscopy
9. b) false, because phase constrast microscope
10. a) true
11. a) true
12. a) true
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