20. Four tripeptide mixtures (I-IV) are separated at pH6.0 using one-dimensional
ID: 175797 • Letter: 2
Question
20.
Four tripeptide mixtures (I-IV) are separated at pH6.0 using one-dimensional high voltage paper electrophoresis
I pro-asp-thr
II lys-ala-val
III tyr-gly-his
IV glu-phe-arg
The predicted order of separation of the peptides, listing first the peptide migrating the closest to the cathode (negative electrode) and last the peptide migrating closest to the anode, is
III,II,I, IV
II, III, IV, I
III, IV, II, I
I, III, II, IV
II, IV, I, III
21.
What is the major purpose for which organisms require energy?
Performance of mechanical work
Active transport
Synthesis of biomolecules
A and C
A, B, and C
22.
Coenzymes that are required by the pyruvate dehydrogenase multienzyme complex are
biotin, CoA, NAD+, pyridoxal phosphate and lipoic acid
thiamine pyrophosphate, tetrahydrofolate, lipoic acid, FAD and CoA
thiamine pyrophosphate, lipoic acid, FAD, CoA and NAD+
FAD, CoA, NAD+, pyrodoxal phosphate and tetrahydrofolate
lipoic acid, FAD, CoA NAD+ and cobalamin
23.
Which of the following conditions will activate pyruvate dehydrogenase kinase, which catalyzes the phorphorylation and inactivation of E1 in the pyruvate dehydrogenase complex?
Elevated concentrations of NADH and ATP
Elevated concentrations of NAD+ and ADP
Ca2+
Insulin
Elevated concentrations of acetyl-CoA
24.
Multifunctional protein kinases
carry out phosphorylation as well as sulfation reactions.
can modify several different targets.
conserve energy in the form of ATP from the degradation of proteins.
All of the above.
None of the above.
III,II,I, IV
II, III, IV, I
III, IV, II, I
I, III, II, IV
II, IV, I, III
Explanation / Answer
20). B.
II, III, IV, I
I pro-asp-thr ------> Charge at pH 6 is, -0.98
II lys-ala-val ----> Charge at pH 6 is, +0.99
III tyr-gly-his ----> Charge at pH 6 is, +0.75
IV glu-phe-arg ---> Charge at pH 6 is, +0.02
As the positively charged molecule move toward the negative electrode, the peptide migrating the closest to the cathode (negative electrode) and last the peptide migrating closest to the anode, is II, III, IV and I.
II, III, IV, I
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