You work in a rural laboratory and until recently, the material for molecular te

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You work in a rural laboratory and until recently, the material for molecular techniques ordered for patient diagnosis was sent to a different laboratory. Your lab just received a thermocycler, so now you should be able to perform some of these tests in house after you develop skills need to use it What assays can you now perform because you have a thermocycler? Name a clinical technique mentioned in chapter 23 that utilizes PCR. Name a clinical technique mentioned in chapter 23 that utilizes RT-PCR.

Explanation / Answer

a. Thermal cycler can be used to detect nucleic acids of any organisms if primers that can specifically amplify sequences of a particular organism or a particular candidate gene are available. Nucleic acids of disease causing bacteria, virus, or any other pathogen can be detected from a sample, using thermocycler.

Any abnormal genes in the body can also be detected using thermal cycler. Quantitation of the pathogens can be done through the number of amplicons obtained in the number of amplification cycles. The more is the number of pathogens, the more is the template size and the less is the number of amplification cycles required.

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You work in a rural laboratory and until recently, the material for molecular te

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