Thank you You are interested in microtubules, and join a lab studying microtubul

Question

Thank you

You are interested in microtubules, and join a lab studying microtubules function and the protein MBP1. MBP1 binds to GDP-bound microtubules. You polymerize microtubules in a small petri dish by adding a purified centrosome, alpha-beta tubulin, and GTP (all which you received from a lab mate). Name a protein found in the centrosome. Explain why you need to add a centrosome to polymerize microtubules. You add MBP1 to your polymerizing microtubules. Describe where you expect MBP1 to bind to the microtubule. (Remember that MBP1 binds to GDP-bound microtubules.) You conduct a different experiment, where you polymerize microtubules in a small dish by adding a purified centrosome, alpha-beta tubulin, and a non-hydrolyzable GTP analogue. Do you expect the microtubules polymerized with a non-hydrolyzable GTP-analog to behave differently compared to the microtubules polymerized with GTP? Explain.

Explanation / Answer

1. Pericentrin is found in centromere.

2. Centrosome is added to polymerise microtubule because of its nucleation of the polymerization of tubulin subunits into the long polymers as centrosome forms the starting point of microtubule growth.

3. Microtubule associated protein binds with tubulin dimers of microtubules. This stabilizes the microtubule structure and encourages polymerization. The detachment of the protein causes the polymerization to destabilize.

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.