You have isolated the gene (XA) that codes for protein X from species A and you

Question

You have isolated the gene (XA) that codes for protein X from species A and you want to determine if a gene encoding an identical protein is present in the genome of species B, i.e. does gene XB exist? What kind of a library would you start with for this experiment? Explain your reasoning. Be sure to include explanation of what distinguishes this type of library from other types, and why other types of libraries would not be appropriate for this study. What techniques would you use to identify the clone(s) of interest?

Explanation / Answer

1. first to sequence the XA gene and retrieve the whole sequence.

2. After sequencing, the observed sequence is searched for any homology in species B by using blast, i.e., in in-slico approach.

3. If no similar result was found, then the particular restriction sites and promoter sequences of the sequenced gene have been identified.

4. The restriction enzymes are used in species B to identify if there is any restriction cuts of desired gene length.

5. If the desired length of the gene is found, then the gene is amplified and cloned with an expression vector to know the protein expression of the gene.

6. The protein expression will be identified and measured and compared the data with the protein X from species A to know the presence of identical protein.

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