1. When producing a recombinant plasmid, which step occurs first ?in the process

Question

1. When producing a recombinant plasmid, which step occurs first ?in the process?

A. cloning wild-type repressor mutant repressor (unable to bind to the operator) ls t repressor (insensitive to lactose) o+ wild-type operator OC constitutive operator (insensitive to repressor) Will a bacterial cell with the following partial hybrid DNA constitution produce any Beta-galactosidase, first in the presence of lactose sugar, and then in the absence of lactose sugar? (he first part below before the slash is the chromosome and the second part after the slash is the plasmid; F' plasmid DNA. I IS O No, no O Yes, no O Not possible to tell O No. yes O Yes, yes

Explanation / Answer

1. Answer is E.

Isolation of plasmid from bacterium followed by cloning.

In the process Recombinant DNA Technology.

2. Answer is 5. Yes, yes

The plasmid is put into the bacterial cell and further it is cloned with gene of interest.

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