b. If we assume that a lagging strand fragment is made from region 1, what will
ID: 174613 • Letter: B
Question
b. If we assume that a lagging strand fragment is made from region 1, what will be its sequence? How Do I know the sequence of the lagging strand?
Answer key attached: Please explain the process.
2. Below is a schematic of a replicating DNA in E.Coli. tee A: Top strand Bottom strand Site BR Region 1 Region 2 a. On the diagram label the 5' and the 3' ends of the parental DNA strands te A: ACA 5' Top strand 3' Bottom strand Site Region 2 Region 1 b. Which parental strand (top or Bottom) will be the template for the leading strand in region 2? Bottom strand c. To which site (A, B or both) the sequence 5' UGUC 3' bind? Site BExplanation / Answer
2.b The bottom strand will be the template for leading strand synthesis because template strand for DNA synthesis read 3'---------->5'. The parent strand of DNA which runs in the 3' to 5' direction toward the fork is called the leading strand.
c site B, because it will be sequence in 3'------>5' direction. The parent strand of DNA which runs in the 3' to 5' direction toward the fork is called the leading strand. For any complete molecule of DNA, one strand will run from 3' to 5', and the other will run from 5' to 3'. Therefore 5'UGUC3' bind with 3'ACAG 5'.
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