1. a)Using the Short Sequence match for the 3’ poly A site (AATAAA) one of the t
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Question
1. a)Using the Short Sequence match for the 3’ poly A site (AATAAA) one of the two matches (figure below) is likely to be the 3’ transcription termination signal for the tra gene? What is your evidence? (Hint: remember which strand contains the tra gene.)
b) What other evidence from the genome browser indicates that this is the 3’ poly A tail signal? (Hint: look at the peak and valleys)
Scale ontigi tra-RB tra-RA 98 31 10,550l 100 bases dm3gene 10,700 10,750 10,800 Perfect Matches to Short Sequence (AATAAA) -10, 784 -10, 818 FlyBase Genes mod ENCODE RNA-Seq from melanogaster Whole Adult Females modENCODE RNA-Seq from D melanogastel Who le ndult Males 10,850Explanation / Answer
There are Three stop codons TAA,TGA, TAG. these sequence contain TAA sequence ,which play as a stop codon in case of TRA gene expression.
Both squence are RNA sequence of D.melanogaster adult male. upstrem +10,818 contains TAAA sequence, which indicates its may be a poly A tail signal.
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