. When transformed into bacteria, which vector would lead to expression of a fus
ID: 174339 • Letter: #
Question
. When transformed into bacteria, which vector would lead to expression of a fusion protein? the vector containing the coding sequences of two proteins located adjacent to one another within a polylinker the vector containing the coding sequences of two proteins; each sequence is located within a separate selectable marker the vector containing the coding sequences of two proteins; one located within a polylinker and one within a selectable marker the vector containing the coding sequences of two proteins; each sequence is mutated so the two proteins will bind when they are expressed the vector containing the coding sequences of two proteins; each sequence is located within a different polylinker . When transformed into bacteria, which vector would lead to expression of a fusion protein? the vector containing the coding sequences of two proteins located adjacent to one another within a polylinker the vector containing the coding sequences of two proteins; each sequence is located within a separate selectable marker the vector containing the coding sequences of two proteins; one located within a polylinker and one within a selectable marker the vector containing the coding sequences of two proteins; each sequence is mutated so the two proteins will bind when they are expressed the vector containing the coding sequences of two proteins; each sequence is located within a different polylinkerExplanation / Answer
A and E.
Normally a vector used in bacteria are of 2kb size, Using of two or more polylinkers are not a good idea for proper expression
We can create a vector with two proteins adjacent to one another within a polylinker, this leads to two proteins which might be intervened in each other. To make these proteins independent, a spacer is introduced in the sequence which will help in independent folding of the proteins
Normally a vector used in bacteria are of 2kb size, Using of two or more polylinkers are not a good idea for proper expression
We can create a vector with two proteins adjacent to one another within a polylinker, this leads to two proteins which might be intervened in each other. To make these proteins independent, a spacer is introduced in the sequence which will help in independent folding of the proteins
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