It is quite common in molecular genetics to add to the amino or carboxy terminus

Question

It is quite common in molecular genetics to add to the amino or carboxy terminus of an expression construct, a short tag of additional amino acids that can be detected with a specific antibody. This is called epitope tagging. Below are the single letter amino acid codes for 5 very common epitope tags Epitope tag peptide sequence Epitope tag name DYKDDDDK Flag YPYDVPDY His DLYDD DDK Myc EQKLISEE Q7. The short nucleotide sequence below would code for which tag? GATTATAAAGATGATGATGATAAA. A. Flag B. HA C. His D. Xpr E. Myc

Explanation / Answer

the answer is that the short nucleotide sequence would code for the Flag. so the answer is option A.

When we convert the DNA sequence using the codon table and the codon to the corresponding amino acid using the single letter code for amino acids we get the sequenceDYKDDDDK. This corresponds to option A which is the correct answer.

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