Answer the following questions from this article: These are the authors G. B. Su

Question

Answer the following questions from this article: These are the authors G. B. Sunil. Kumar Æ T. R. Ganapathi Æ C. J. Revathi L. Srinivas Æ V. A. Bapat This is the title Expression of hepatitis B surface antigen in transgenic banana plants

1.How do the authors propose to vaccine humans with bananas? 2.Why do they show two figures with PCR amplification products (Fig. 4 and 5) and how do these results differ from what is presented in Figure 6? 3.Why did they run a RT-PCR in addition to the aforementioned two PCR reactions? How is RT-PCR different from PCR? 4.What is the point of the ELISA analysis and the buoyant density analysis?

Explanation / Answer

Hepatitis B is the most serious type of liver infection caused by hepatitis B virus but it can be prevented by Recombinant vaccines which are produced by using bacteria east mammalian and plant cells. It should be known is circular molecule of 3.2 KB

The embryonic cells of Banana are Transformer with the s gene of hepatitis B surface antigen using agrobacterium. The transgenic nature of the plants and expression of antigen is confirmed by the techniques like Southern hybridization and polymerase chain reaction. The transgenic plants are grown in the green houses and the expression of the antigen is confirmed by RT PCR this plants are multiplied in vitro conditions.

The hbs antigen was first expressed in transgenic banana fruit in India in 2005. Taryn Terrell vaccination with purifier plant produced hbs antigen followed by oral boosting with left purified antigen as a capsules is prove to be effective.

The authers explain thath plant based oral hepatitis b vaccine is economic and has many advantages over injectable vaccines.

The buoyant density in cscl of hbs antigen derived from transgenic banana leaves was found to be 1.146 gram per ml which is similar to human serum derived one in buoyant density properties. Attempts for being done to improve the expression of the antigen in the transgenic banana plant leaves by wounding or treating with plant growth regulators.

Molecular analysis of transgenic Banana plants.- in order to observe the hbs antigen expressed Elisa analysis is used analysis total protein was extracted from non transformed control and transformed cell lines of banana leaves the fractions obtained after ultracentrifugation assayedid for the levels of expression of the antigen.

Integration of D transgene was confirmed by PCR technique isolated from transformed and control cells

Two figures of PCR analysis show the transformed tobacco cell lines on the banana plants.

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