To measure 15 microliters which of the following would you use? 100-1000 micropi

Question

To measure 15 microliters which of the following would you use? 100-1000 micropipette 1-10 micropipette 2-20 micropipette 5 mL serological pipet You wish to separate two proteins by gel filtration chromatography, horse myoglobin (molecular weight is 17,000 Daltons) and cow hemoglobin (molecular weight is 64,000 dollars). Which proteins do you expect to evaluate first? Cow hemoglobin horse myoglobin both will elute at the same time neither will elute 5 milliliters (mL) is the same volume as which of the following? 5 microliters 50 microliters 50 microliters 5000 microliters. In get filtration chromatography, which of the following constitutes the mobile phase? Elution buffer Agarose Microscopic polymer beads Ion-exchange resin

Explanation / Answer

2.Ans. Cow hemoglobin will elute first. The reason is Gel filtration chromatography separates proteins, peptides, on the basis of size. Molecules move through a bed of porous beads, diffusing into the beads to greater or lesser degrees. Smaller molecules diffuse further into the pores of the beads and therefore move through the bed more slowly, while larger molecules enter less or not at all and thus move through the bed more quickly. Here cow hemoglobin is bigger thn horse myoglobin. So ans is a.

1. The microtips generally used in the different processes are of different capacities. Microtips that have range of 2-20 microlitre should be used. So ans is c.

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