Assume that the previous experiment was repeated with a filter placed between th

Question

Assume that the previous experiment was repeated with a filter placed between the E. coli strains that prevented them from contacting one another, but allowed material smaller than the cells to pass between strains (the Davis U-tube). What would be the mode of recombination if no prototrophs were produced on either side of the filter. Assume that the previous experiment was repeated with a filter placed between the E. coli strains that prevented them from contacting one another, but allowed material smaller than the cells to pass between strains (the Davis U-tube). What would be the mode of recombination if prototrophs were produced on one side of the filter (from one strain).

Explanation / Answer

Answer:

The experiment referred to here is probably the Davis U-tube experiment.

9. The filter allows small particles (smaller than bacterial cells) to pass through. This will also allow naked DNA to cross the barrier and reach the either side of the filter.

Since no prototrophs were produced, the mode of recombination requires physical contact. So, the mode of recombination is conjugation (which occurs through conjugal tube formation between the two strains of bacteria).

10. Since prototrophs were produced, it does not require physical contact between the two strains of bacteria. The mode of recombination is transformation which can occur through uptake of naked DNA by bacteria.

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