In studying the bacterial the srp operon, you have isolated two different mutant
ID: 172267 • Letter: I
Question
In studying the bacterial the srp operon, you have isolated two different mutants (con1 and con2) that cause constitutive expression of the srp, operon (constitutive expression of srp1 and srp2). One of the mutants has a mutation in a DNA binding site (e.g. operator), and the other has a loss-of-function mutation in the gene that encodes a protein that binds to the DNA binding site (e.g. repressor protein). You perform an analysis using partial diploids to determine which of the two mutants has a defect in the binding site and which of the two mutants has a mutation in the gene encoding the binding protein. Assuming you can assay levels of the two Srp proteins, what results do you predict for the two strains (i and ii below) if con2 is the regulatory binding site and con1 encodes the regulatory protein? In predicting results, indicate whether expression of the Srp1 and Srp2 proteins will be constitutive or inducible (you will circle an answer in the bolded region below). i. con1^-con2^+srpl-srp^2+/con1^+con2^-srp1^+srp2^- ii. con1^+ con2^2 srp1 - srp2^+/con1^+con2^- srp1^+ srp2^- Answer for strain i (circle either constitutive or inducible for each gene): Answer for strain ii (circle either constitutive or inducible for each gene):Explanation / Answer
Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product.Regulation of gene expression refers to the control of the amount and timing of appearance of the functional product of a gene. Control of expression is vital to allow a cell to produce the gene products it needs when it needs them; in turn, this gives cells the flexibility to adapt to a variable environment, external signals, damage to the cell, and other stimuli. More generally, gene regulation gives the cell control over all structure and function, and is the basis for cellular differentiation, morphogenesis and the versatility and adaptability of any organism.
Constitutive genes: A gene that is transcribed at a relatively constant level regardless of the cell environmental conditions. Supplement. Unlike facultative genes that are transcribed only when needed, constitutive genes are expressed continuously
Inducible gene: A gene whose expression is either responsive to environmental change or dependent on the position in the cell cycle.
For Strain i:
srp1=Constitutive expression
srp2= Inducible expression
For Strain ii:
srp1=Constitutive expression
srp2= Constitutive expression
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