where the pencil is pointing is where my bands for the undigested insert and the

Question

where the pencil is pointing is where my bands for the undigested insert and the insert digested with BamHI are. both of them are about the same size. is this because I messed up and my results are faulty or because neither of them were cut or both of them were cut? One has the PCR fragment enzyme and the other has the PCR fragment and BamHI.

e order; be. N ATE pip a evel st ubes on the caps IVC", VR "IC", "IR" (C contro of the tubes label them with your table tt and section a volume of water needed in each tube --4 he then last, add DN 3 20

Explanation / Answer

To know whether your results are correct and if your gene of interest is uncut, compare the molecular weight of your PCR product with your sample of digestion, If the molecular weight is similar, it means the restriction of the enzyme did not occur.

Try to predict the molecular weight of the protein with known sequence using online digesters to know the exact molecular weight to be obtained.

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