With respect to the wild-type sequence below, identify the mutation in each alte

Question

With respect to the wild-type sequence below, identify the mutation in each altered sequence as one of the following types: missense mutation, nonsense mutation, frameshift mutation, reverse mutation, intragenic suppressor mutation, or intergenic suppressor mutation. For each altered sequence, more than one answer is possible. This DNA sequence encodes the last amino acids of a protein that is 270 amino acids long. The first three bolded base pairs indicate the frame and include the coding region. Inserted nucleotides are italicized. Wild type:
5...GCTAAGTATTGCTCAAGATTAGGATGATAAATAACTGG3
3...CGATTCATAACGAGTTCTAATCCTACTATTTATTGACC5

Part A

Altered sequence 1:
5...GCTAAGTATTGCTCAACCGATTAGGATGATAAATAACTGG3
3...CGATTCATAACGAGTTGGCTAATCCTACTATTTATTGACC5

Check all that apply.

- intragenic suppressor mutation

- intergenic suppressor mutation

Part B

Altered sequence 2--changing altered sequence 1:
5...GCTAAGTATTGCTCACCGATTAGGATGATAAATAACTGG3
3...CGATTCATAACGAGTGGCTAATCCTACTATTTATTGACC5

Check all that apply.

- nonsense mutation

- missense mutation

Part C

Altered sequence 3--changing altered sequence 1:
5...GCTAAGTATTGCTCCCGATTAGGATGATAAATAACTGG3
3...CGATTCATAACGAGGGCTAATCCTACTATTTATTGACC5

Check all that apply.

- nonsense mutation

- missense mutation - frameshift mutation - reverse mutation - nonsense mutation

- intergenic suppressor mutation

Part B

Altered sequence 2--changing altered sequence 1:
5...GCTAAGTATTGCTCACCGATTAGGATGATAAATAACTGG3
3...CGATTCATAACGAGTGGCTAATCCTACTATTTATTGACC5

Check all that apply.

- nonsense mutation

- frameshift mutation - intragenic suppressor mutation - reverse mutation - intergenic suppressor mutation

- missense mutation

Part C

Altered sequence 3--changing altered sequence 1:
5...GCTAAGTATTGCTCCCGATTAGGATGATAAATAACTGG3
3...CGATTCATAACGAGGGCTAATCCTACTATTTATTGACC5

Check all that apply.

- nonsense mutation

- frameshift mutation - missense mutation - intragenic suppressor mutation - reverse mutation - intergenic suppressor mutation

Explanation / Answer

Part 1.

GCT AAG TAT TGC TCA AGA TTA GGA TGA TAA ATA ACT GG

Code for

A K Y C S R L G Stop Stop I T

GCT AAG TAT TGC TCA ACC GAT TAG GAT GAT AAA TAA CTG G

Code for

A K Y C S T D Stop D D K Stop L

As we can see that the entire frame has shifted due to insertion of two Cytosine which will code for new amino acids apart from that due to frameshift one amino acid codon is changed into stop codon

Frameshift mutation and a nonsense mutation.

Part B.

GCT AAG TAT TGC TCA ACC GAT TAG GAT GAT AAA TAA CTG G

Code for

A K Y C S T D Stop D D K Stop L

GCT AAG TAT TGC   TCA CCG   ATT AGG ATG ATA   AAT   AAC TGG

Codes for

A K Y C S P I R Met I N N W

As we can see that the entire frame has shifted due to insertion of Adenine due to which stop codons changed into amino acid coding codon and now codes for the new peptide which will be longer than the given peptide

Frameshift mutation.

Part C.

Part B.

GCT AAG TAT TGC TCA ACC GAT TAG GAT GAT AAA TAA CTG G

Code for

A K Y C S T D Stop D D K Stop L

GCT AAG TAT TGC TCC CGA TTA GGA TGA TAA ATA ACT GG

Code for

A K Y C S R L G Stop Stop I T

As we can see that the entire frame has shifted of due to deletion of two Adenine from the sequence due to which one new amino acid is introduced in the protein sequence. New sequence will code for same protein that was coded by the wild type.

Frameshift mutation and intragenic suppressor mutation (Due to mutation wild type sequence has created

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