How to find the concentration of reduced cytochrome c when you know the extincti

Question

How to find the concentration of reduced cytochrome c when you know the extinction coefficient, the cuvette size, and the OD at 550?
The extinction coefficient is 29.5 for reduced cytochrome c OD was 0.91 AU The cuvette size was a 1 cm x 1 cm There was 1 milliliter of cytochrome c in the cuvette when it was measured
Please show the math of how you come up with the answer. How to find the concentration of reduced cytochrome c when you know the extinction coefficient, the cuvette size, and the OD at 550?
The extinction coefficient is 29.5 for reduced cytochrome c OD was 0.91 AU The cuvette size was a 1 cm x 1 cm There was 1 milliliter of cytochrome c in the cuvette when it was measured
Please show the math of how you come up with the answer.
The extinction coefficient is 29.5 for reduced cytochrome c OD was 0.91 AU The cuvette size was a 1 cm x 1 cm There was 1 milliliter of cytochrome c in the cuvette when it was measured
Please show the math of how you come up with the answer.

Explanation / Answer

Answer:

Beer lambert's law:

A= ECl

A= OD= 0.91 Arbitrary units

E=29.5 L mol-1 cm-1

C=?

l=length of the cuvette= 1cm

Rearranging the formula

A= ECl

C=A/El

C= 0.91/29.5

C=0.030 L mol-1 cm-1

concentration of reduced cytochrome c is 0.030 L mol-1 cm-1

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