the question is (1)The above data is plotted as double reciprocal (Lineweaver-Bu

Question

the question is

(1)The above data is plotted as double reciprocal (Lineweaver-Burke) plots. The y-intercept (ie. The value of y when x = 0) is

a.      Larger for enzyme

b.      Larger for enzyme

c.      The same for both enzme

d.      A negative number

(2)In the Michaeli-Menten model of enzyme kinetics the parameter which is most closely related to the binding of the substrate to the enzyme is

         a.      Km--> i think this is the correct answer

         b.      Vmax

         c.      [S]

         d.      [P]

         e.      None of the above is related to enzyme-substrate binding

(3) Active transport always involves

         a.      A charged species

         b.      A protein carrier

         c.      H+

         d.      G > 0 --> i think the answer is this since it'll be endergonic

         e.      ATP synthesis

(4) When there is a difference in concentration of a charged species (ie. An ion) across a membrane there is also a difference in

         a.      pH

         b.      Electrical potential (voltage)--> i think the asnwe would be this one

         c.      H+

         d.      ATP concentration

         e.      All of the above

(5) The interior region of a lipd bilayer is usually

         a.      Positively charged

         b.      Negatively charged

         c.      Hydrophilic

         d.      Acidic

         e.      None of the above

Two different enzymes can catalyze the same reaction, A B. The reaction rate (v) for each of the enzymes was measured as a function of the substrate concentration LA], and the following results were obtained: [A] Enzyme 1 Enzyme 2 33 16.7 25 96 50 48 100 98 49 200 99 50 500 50 1000 100 50 100 50 2000

Explanation / Answer

2). A. a.      Km-.

3) A protein carrier.

Active transport is carried by membrane proteins called carriers.

4. B. Electrical potential.

Electrical potential across membrane is maintained due to difference in electrical charge on the two sides of membrane.

5). E. None of these.

Interior region of lipid bilayer is made of fatty acid tails which are hydrophobic. Hydrophobic molecules, are insoluble in water because all, or almost all, of their atoms are uncharged and nonpolar and therefore cannot form energetically favorable interactions with water molecules.

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