Fragment Number Size (bp) 1 1.20 2 - 3 2.45, 1.27, 0.4 4 2.48, 1.30, 0.4 5 2.45,

Question

Fragment Number

Size (bp)

1

1.20

2

-

3

2.45, 1.27, 0.4

4

2.48, 1.30, 0.4

5

2.45, 1.17

R = RNA sample

DR =DNAse treated RNA

RT-R = Reverse Transcriptase RNA sample

RT-DR = Reverse Transcriptase DNAse treated RNA

- all of these samples undergo PCR

What does the difference in size between the PCR products tell you about the gene from which this RNA is derived from?

Fragment Number

Size (bp)

1

1.20

2

-

3

2.45, 1.27, 0.4

4

2.48, 1.30, 0.4

5

2.45, 1.17

M 1 2 3 4 5 Reaction Template PCR-1 R (Step I) PCR-2 LDR (step II. PCR-3 RT-R (step III PCR-4 RT-DR (step III) PCR-5 Yeast genomic DN

Explanation / Answer

The difference in the size indicates that the DNA is amplified several times as this technique itself is for amplication of DNA . The different bands for the same RNA indicates there occurs different splicing event for the same RNA. Alternative splicing is a process in which by alternative removal of different introns produces different proteins. Hence different bands in PCR .

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